Serendipity Symposium 2023 focuses on technologies and applications of next-generation cytometry. Topics covered in the symposium include, but are not limited to, the high-throughput, high-content, and high-dimensional aspects of diverse cytometry technologies (e.g., fluorescence microscopy, Raman spectroscopy, mass spectrometry, super-resolution imaging, microfluidics, deep learning) as well as a novel class of cytometric applications enabled by the technologies. The symposium will provide a valuable forum where researchers and engineers in both academic and industrial sectors will be able to share and discuss their most recent findings and understandings of basic principles, advanced techniques, and applications of next-generation cytometry.